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dermal fibroblast hs68 cell line  (ATCC)


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    ATCC dermal fibroblast hs68 cell line
    Dermal Fibroblast Hs68 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 613 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dermal+fibroblast+cell+line+hs68/Hs68/pmc12732660-164-2-7
    Average 96 stars, based on 613 article reviews
    dermal fibroblast hs68 cell line - by Bioz Stars, 2026-09
    96/100 stars

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    Cell Culture:

    Article Title: α-Ionone alleviates chronic UVB exposure-induced skin photoaging in mice by remodeling the gut microbiota
    Article Snippet: .. The human dermal fibroblast cell line Hs68 (ATCC, USA) was cultured in DMEM containing 10% FBS and 1% penicillin-streptomycin at 37°C with 5% CO2. ..

    Article Title: Improvement in Facial Wrinkles Using Materials Enhancing PPARGC1B Expression Related to Mitochondrial Function
    Article Snippet: .. The human dermal fibroblast cell line Hs68 (ATCC, Manassas, VA, USA) was cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco, Waltham, MA, USA) supplemented with 10% ( v / v ) fetal bovine serum (FBS; Gibco, Waltham, MA, USA) and penicillin-streptomycin (Gibco, Waltham, MA, USA) at 37 °C in an atmosphere with 5% CO 2 . .. NAM (pyridine-3-carboxamide), CAM (1,7,7-trimethylbicyclo [2.2.1]heptan-2-one), P-HCl, TNA, and retinol were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Article Title: Multifunctional Dermatological Effects of Whole-Plant Bassia scoparia Extract: Skin Repair and Protection
    Article Snippet: .. The human dermal fibroblast cell line Hs68 (ATCC, Manassas, VA, USA) was cultured in DMEM supplemented with 10% FBS and 1% antibiotic–antimycotic at 37 °C in a humidified incubator with 5% CO 2 . ..

    Article Title: Ginsenosides C-Mc1 and C-O Unique to Wild Ginseng: Novel Senotherapeutic Agents to Regulate Cellular Senescence
    Article Snippet: .. The human dermal fibroblast cell line Hs68 (ATCC, Manassas, VA, USA) was cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco, Waltham, MA, USA) supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, Waltham, MA, USA) and 1% (v/v) penicillin–streptomycin (PS; Gibco, Waltham, MA, USA) and incubated at 37 °C with 5% CO2. .. To induce senescence, fibroblasts were irradiated once with UV-B light (30 Watt, 312 nm) at an intensity of 20 mJ/cm2 using BIO-SUN irradiation system (Vilber Lourmat, Marne-la-Vallée, France).

    Modification:

    Article Title: Improvement in Facial Wrinkles Using Materials Enhancing PPARGC1B Expression Related to Mitochondrial Function
    Article Snippet: .. The human dermal fibroblast cell line Hs68 (ATCC, Manassas, VA, USA) was cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco, Waltham, MA, USA) supplemented with 10% ( v / v ) fetal bovine serum (FBS; Gibco, Waltham, MA, USA) and penicillin-streptomycin (Gibco, Waltham, MA, USA) at 37 °C in an atmosphere with 5% CO 2 . .. NAM (pyridine-3-carboxamide), CAM (1,7,7-trimethylbicyclo [2.2.1]heptan-2-one), P-HCl, TNA, and retinol were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Article Title: Ginsenosides C-Mc1 and C-O Unique to Wild Ginseng: Novel Senotherapeutic Agents to Regulate Cellular Senescence
    Article Snippet: .. The human dermal fibroblast cell line Hs68 (ATCC, Manassas, VA, USA) was cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco, Waltham, MA, USA) supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, Waltham, MA, USA) and 1% (v/v) penicillin–streptomycin (PS; Gibco, Waltham, MA, USA) and incubated at 37 °C with 5% CO2. .. To induce senescence, fibroblasts were irradiated once with UV-B light (30 Watt, 312 nm) at an intensity of 20 mJ/cm2 using BIO-SUN irradiation system (Vilber Lourmat, Marne-la-Vallée, France).

    Incubation:

    Article Title: Ginsenosides C-Mc1 and C-O Unique to Wild Ginseng: Novel Senotherapeutic Agents to Regulate Cellular Senescence
    Article Snippet: .. The human dermal fibroblast cell line Hs68 (ATCC, Manassas, VA, USA) was cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco, Waltham, MA, USA) supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, Waltham, MA, USA) and 1% (v/v) penicillin–streptomycin (PS; Gibco, Waltham, MA, USA) and incubated at 37 °C with 5% CO2. .. To induce senescence, fibroblasts were irradiated once with UV-B light (30 Watt, 312 nm) at an intensity of 20 mJ/cm2 using BIO-SUN irradiation system (Vilber Lourmat, Marne-la-Vallée, France).



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    dermal fibroblast hs68 cell line  (ATCC)
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    ATCC dermal fibroblast hs68 cell line
    Dermal Fibroblast Hs68 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hs68 human dermal fibroblast cell line  (ATCC)
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    Effects of 10E-PDA on inhibiting melanogenesis in α-MSH-stimulated B16F10 melanoma cells. ( a ) B16F10, ( b ) <t>HS68,</t> and ( c ) HaCaT cells were treated with increasing concentrations of 10E-PDA (1–100 μM) for 24 h, after which cell viability was assessed (n = 4 per group). ( d , e ) B16F10 cells were pretreated with different concentrations of 10E-PDA (1–15 μM) or kojic acid (30 μM) for 1 h, followed by exposure to α-MSH (500 nM) for 6 days to measure melanin content ( d ) or for 3 days to examine tyrosinase activity ( e ). In both panels, the white bar represents the untreated control group without α-MSH stimulation, the black bar represents the α-MSH-stimulated control group, and the dark gray bars indicate the 10E-PDA-treated groups under α-MSH stimulation. In panel ( e ), the light gray bar represents the positive control group treated with kojic acid under α-MSH stimulation. Data are shown as mean ± SEM. ### p < 0.001 compared with the untreated control group, and * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the α-MSH-treated group.
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    human diploid dermal fibroblast hs68 cell line  (BioResource International Inc)
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    Changes in <t>Hs68</t> cell characteristics in the presence of CAP. Hs68 cell characteristics were examined following treatment of cells with different CAP concentrations (A) Cell viability, (B) migration (cells were also treated with MMC and TNF-α) and (C) percentage closure based on the cell migration assay were determined. (D) Relative mRNA levels of IL-6 in Hs68 cells treated with TNF-α and CAP. Scale bar, 500 µm. **P<0.01, ****P<0.0001. CAP, capsaicin; IL-6, interleukin 6; MMC, mitomycin C; TNF-α, tumor necrosis factor-α.
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    human diploid dermal fibroblast hs68 cell line (passage 19)  (BioResource International Inc)
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    BioResource International Inc human diploid dermal fibroblast hs68 cell line (passage 19)
    Changes in <t>Hs68</t> cell characteristics in the presence of CAP. Hs68 cell characteristics were examined following treatment of cells with different CAP concentrations (A) Cell viability, (B) migration (cells were also treated with MMC and TNF-α) and (C) percentage closure based on the cell migration assay were determined. (D) Relative mRNA levels of IL-6 in Hs68 cells treated with TNF-α and CAP. Scale bar, 500 µm. **P<0.01, ****P<0.0001. CAP, capsaicin; IL-6, interleukin 6; MMC, mitomycin C; TNF-α, tumor necrosis factor-α.
    Human Diploid Dermal Fibroblast Hs68 Cell Line (Passage 19), supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of 10E-PDA on inhibiting melanogenesis in α-MSH-stimulated B16F10 melanoma cells. ( a ) B16F10, ( b ) HS68, and ( c ) HaCaT cells were treated with increasing concentrations of 10E-PDA (1–100 μM) for 24 h, after which cell viability was assessed (n = 4 per group). ( d , e ) B16F10 cells were pretreated with different concentrations of 10E-PDA (1–15 μM) or kojic acid (30 μM) for 1 h, followed by exposure to α-MSH (500 nM) for 6 days to measure melanin content ( d ) or for 3 days to examine tyrosinase activity ( e ). In both panels, the white bar represents the untreated control group without α-MSH stimulation, the black bar represents the α-MSH-stimulated control group, and the dark gray bars indicate the 10E-PDA-treated groups under α-MSH stimulation. In panel ( e ), the light gray bar represents the positive control group treated with kojic acid under α-MSH stimulation. Data are shown as mean ± SEM. ### p < 0.001 compared with the untreated control group, and * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the α-MSH-treated group.

    Journal: Antioxidants

    Article Title: 10(E)-Pentadecenoic Acid Inhibits Melanogenesis Partly Through Suppressing the Intracellular MITF/Tyrosinase Axis

    doi: 10.3390/antiox13121547

    Figure Lengend Snippet: Effects of 10E-PDA on inhibiting melanogenesis in α-MSH-stimulated B16F10 melanoma cells. ( a ) B16F10, ( b ) HS68, and ( c ) HaCaT cells were treated with increasing concentrations of 10E-PDA (1–100 μM) for 24 h, after which cell viability was assessed (n = 4 per group). ( d , e ) B16F10 cells were pretreated with different concentrations of 10E-PDA (1–15 μM) or kojic acid (30 μM) for 1 h, followed by exposure to α-MSH (500 nM) for 6 days to measure melanin content ( d ) or for 3 days to examine tyrosinase activity ( e ). In both panels, the white bar represents the untreated control group without α-MSH stimulation, the black bar represents the α-MSH-stimulated control group, and the dark gray bars indicate the 10E-PDA-treated groups under α-MSH stimulation. In panel ( e ), the light gray bar represents the positive control group treated with kojic acid under α-MSH stimulation. Data are shown as mean ± SEM. ### p < 0.001 compared with the untreated control group, and * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the α-MSH-treated group.

    Article Snippet: The B16F10 murine melanoma cell line and Hs68 human dermal fibroblast cell line were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

    Techniques: Activity Assay, Control, Positive Control

    Changes in Hs68 cell characteristics in the presence of CAP. Hs68 cell characteristics were examined following treatment of cells with different CAP concentrations (A) Cell viability, (B) migration (cells were also treated with MMC and TNF-α) and (C) percentage closure based on the cell migration assay were determined. (D) Relative mRNA levels of IL-6 in Hs68 cells treated with TNF-α and CAP. Scale bar, 500 µm. **P<0.01, ****P<0.0001. CAP, capsaicin; IL-6, interleukin 6; MMC, mitomycin C; TNF-α, tumor necrosis factor-α.

    Journal: Molecular Medicine Reports

    Article Title: Improvement of wound healing by capsaicin through suppression of the inflammatory response and amelioration of the repair process

    doi: 10.3892/mmr.2023.13042

    Figure Lengend Snippet: Changes in Hs68 cell characteristics in the presence of CAP. Hs68 cell characteristics were examined following treatment of cells with different CAP concentrations (A) Cell viability, (B) migration (cells were also treated with MMC and TNF-α) and (C) percentage closure based on the cell migration assay were determined. (D) Relative mRNA levels of IL-6 in Hs68 cells treated with TNF-α and CAP. Scale bar, 500 µm. **P<0.01, ****P<0.0001. CAP, capsaicin; IL-6, interleukin 6; MMC, mitomycin C; TNF-α, tumor necrosis factor-α.

    Article Snippet: The human diploid dermal fibroblast Hs68 cell line (passage 19), which is frequently used in dermal research and has the characteristics of primary dermal fibroblasts , was purchased from the Bioresource Collection and Research Center in the Food Industry Research and Development Institute (Hsinchu, Taiwan).

    Techniques: Migration, Cell Migration Assay